Iron in Human Serum with Roche c502

Detection of Iron in Human Serum with Roche c502

Test Name: In vitro test for the quantitative determination of iron in human serum and plasma on Roche/Hitachi cobas c systems.
Method Name: Colorimetric assay. Ascorbate reduces Fe3+ ions to Fe2+ ions, which then react with FerroZine to form a colored complex. The color intensity is directly proportional to the iron concentration and can be measured photometrically.
Results: Technical Range: 5-1000 µg/dL
Reportable Range: 5-694.4 µg/dL
Reference Ranges: Taken from Mayo Clinic
Males: 50-150 µg/dL
Females: 35-145 µg/dL
Clinical Significance: Ingested iron is mainly absorbed in the form of Fe2+ in the duodenum and upper jejunum. The trivalent form and the heme bound Fe3+ component of iron in food has to be reduced by vitamin C. About 1 mg of iron is assimilated daily. Upon reaching the mucosal cells, Fe2+ ions become bound to transport substances. Before passing into the plasma, these are oxidized by ceruloplasmin to Fe3+ and bound to transferrin in this form. The transport of Fe ions in blood plasma takes place via transferrin iron complexes. A maximum of 2 Fe3+ ions per protein molecule can be transported. Serum iron is almost completely bound to transferrin.

Iron (non heme) measurements are used in the diagnosis and treatment of diseases such as iron deficiency anemia, hemochromatosis (a disease associated with widespread deposit in the tissue of the two iron containing pigments, hemosiderin and hemofuscin, and characterized by pigmentation of the skin), and chronic renal disease. Iron determinations are performed for the diagnosis and monitoring of microcytic anemia (e.g., due to iron metabolism disorders and hemoglobinopathy), macrocytic anemia (e.g., due to vitamin B12 deficiency, folic acid deficiency and drug induced metabolic disorders of unknown origin) as well as normocytic anemias such as renal anemia (erythropoetin deficiency), hemolytic anemia, hemoglobinopathy, bone marrow disease and toxic bone marrow damage.

Submission Criteria: For specimen collection and preparation, only use suitable tubes or collection containers.
Only the specimens listed below were tested and found acceptable.
Plasma: Li-heparin
Do not use EDTA or oxalate plasma.

The sample types listed were tested with a selection of sample collection tubes that were commercially available at the time of testing, therefore not all available tubes of all manufacturers were tested. Sample collection systems from various manufacturers may contain differing materials which could affect the test results in some cases.  When processing samples in primary tubes (sample collection systems), follow the instructions of the tube manufacturer.

Storage and Stability:    3 years at -20°C
21 days at 2-8°C
7 days at 20-25 °C

Rejection Criteria: Rejection criteria include but are not limited to: Specimens containing fibrin or clots. Excessive platelet clumping Leaking specimensSubstandard mixing or collection Expired or improperly stored collection tubes.Improperly filled tubes based on collection tube manufacturer’s guidelines.Contaminated specimens (IV fluid, foreign particles, etc.) Specimens not analyzed within the appropriate time frame.Samples not shipped at appropriate temperature.Samples without 2 proper identifiers or samples having identifiers that do not match the electronic or paper lab requisition.
Authorization: Diagnostic testing can only be performed with approval from an authorized provider/agency.
Turn Around Time: 1 day.