Aerobic and Anaerobic Wound Culture

Aerobic and Anaerobic Wound Culture

Test Name: In vitro test for the quantitative determination of aerobic wound
culture with gram stain.
Method Name: Culture and Sensitivity with Gram stain
Platform: Bruker MALDI-TOF; BD Phoenix
Reference Ranges: No growth, Normal Skin Microbiota; Identification of probable
Clinical Significance: Anaerobic bacteria area significant component of the normal
microbiota of the human host. There are anaerobes on most body
surfaces and mucous membranes; they exist in large numbers
throughout the entire gastrointestinal tract from mouth to colon.
Anaerobes can cause infection when they are introduced into a new
site in the body (endogenous infections) or when nonnormal
microbiota anaerobic bacterium gains entrance into the host.
Submission Criteria: Specimen source must be included on the specimen label as well as
date and time of collection. Specimen must arrive within 48 hours
of collection. Preferably collect specimen prior to institution of

Swab/Container: ESwab with ESwab transport medium (Amies

Storage and Stability:

Specimen                            Stability at 20-25°C                            Stability at 2-8°C                              Stability at <-20°C Collection Media                   Room Temp.                                  Refrigerated Temp.                                      Frozen ESwab (Amies Buffer)             48 Hours                                             48 Hours                                            Unacceptable

Rejection Criteria: Rejection criteria include but are not limited to:
1. Specimens submitted with mismatched, incorrect, or blank
requisition forms.
2. Leaking specimens.
3. Substandard mixing or collection.
4. Expired or improperly stored collection tubes
5. Improperly filled tubes based on collection tube
manufacture’s guidelines.
6. Specimens which arrive at the lab past the 48-hour mark
7. Specimens submitted without a signed patient consent for
treatment prior to specimen collection.
8. Samples not shipped or stored at appropriate temperature.
9. Samples without two proper identifiers or samples having
identifiers that do not match the electronic or paper lab
Authorization: Diagnostic testing can only be performed with approval from an authorized provider/agency.
Turn Around Time: 3-5 Business Days. Test may be delayed if repeat testing or send out
testing is required.